Charly is a senior Biology student who is also pursuing minors in Chemistry and Psychology.\u00a0 This summer, she worked with Dr. Zachary Murphy through a sponsorship with the Summer Fellows Research Program on, “Investigating the Role of TET2 in Erythropoiesis with CRISPR\/Cas9,”<\/p>\n
Charly described her research: “<\/span>Erythropoiesis is a biological process that involves the production of erythrocytes, <\/span>commonly known as red blood cells. This process is crucial to human beings because <\/span>the molecules that interact with the red blood cells help to transport oxygen to all areas\u00a0<\/span>of the body. Red blood cells must take on a biconcave morphology so that the specific\u00a0<\/span>interactions between proteins and molecules can be made. This physical characteristic\u00a0<\/span>is created by the removal of the cell\u2019s nucleus and other cellular components during the\u00a0<\/span>developmental stage. If this developmental process is disrupted in any way, the cell will\u00a0<\/span>not be able to carry the normal amount of oxygen. These disruptions can lead to\u00a0<\/span>diseases such as anemia and myelodysplastic syndromes (MDS). One of the human\u00a0<\/span>genes, TET2, has been suggested to be related to MDS. It is thought that TET2 could\u00a0<\/span>play a role in the development of red blood cells, which could mean that a lack or\u00a0<\/span>mutation of the gene could lead to improper cells being produced. Our research has\u00a0<\/span>looked at this gene and what happens to cells when CRISPR\/Cas9 technology is\u00a0<\/span>introduced along with specific short guide RNA (sgRNA) sequences . The sgRNA\u00a0<\/span>sequences were designed to target exons 3, 7, and 11 of the gene. Our hypothesis is\u00a0<\/span>that if these three exons are targeted and mutated, then the cells will not take on the\u00a0<\/span>proper shape needed.<\/span><\/p>\n “Alongside this idea, our research has also looked into finding inexpensive <\/span>alternatives to protocols and biochemical materials. Science can be quite costly, and\u00a0<\/span>this can create a financial barrier to students and schools that choose to work with\u00a0<\/span>erythroid research. We tested various protocols several times to determine whether or\u00a0<\/span>not these alternatives would be suitable to use for future experiments. This tied into the\u00a0<\/span>research above because this idea of finding alternatives came about while in the\u00a0<\/span>process of troubleshooting. Our goal is to create cost effective alternatives so that other\u00a0<\/span>institutions, like St. John Fisher, can conduct erythroid research. This could help make\u00a0<\/span>research more diverse and bring in more ideas that could change the world of erythroid <\/span>research.”<\/span><\/p>\n","protected":false},"excerpt":{"rendered":" Charly is a senior Biology student who is also pursuing minors in Chemistry and Psychology.\u00a0 This summer, she worked with Dr. Zachary Murphy through a sponsorship with the Summer Fellows Research Program on, “Investigating the Role of TET2 in Erythropoiesis with CRISPR\/Cas9,” Charly described her research: “Erythropoiesis is a biological process that involves the production … Continue reading “Student Summer Research – Charly Campanella”<\/span><\/a><\/p>\n","protected":false},"author":183,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/projects.sjf.edu\/cangie\/wp-json\/wp\/v2\/posts\/135"}],"collection":[{"href":"https:\/\/projects.sjf.edu\/cangie\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/projects.sjf.edu\/cangie\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/projects.sjf.edu\/cangie\/wp-json\/wp\/v2\/users\/183"}],"replies":[{"embeddable":true,"href":"https:\/\/projects.sjf.edu\/cangie\/wp-json\/wp\/v2\/comments?post=135"}],"version-history":[{"count":2,"href":"https:\/\/projects.sjf.edu\/cangie\/wp-json\/wp\/v2\/posts\/135\/revisions"}],"predecessor-version":[{"id":145,"href":"https:\/\/projects.sjf.edu\/cangie\/wp-json\/wp\/v2\/posts\/135\/revisions\/145"}],"wp:attachment":[{"href":"https:\/\/projects.sjf.edu\/cangie\/wp-json\/wp\/v2\/media?parent=135"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/projects.sjf.edu\/cangie\/wp-json\/wp\/v2\/categories?post=135"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/projects.sjf.edu\/cangie\/wp-json\/wp\/v2\/tags?post=135"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}